An Inactivated Novel Trivalent Vaccine Provides Complete Protection against FAdV-4 Causing Hepatitis-Hydropericardium Syndrome and FAdV-8b/-11 Causing Inclusion Body Hepatitis

Outbreaks of hepatitis-hydropericardium syndrome (HHS) caused by fowl adenovirus serotype 4 (FAdV-4) and inclusion body hepatitis (IBH) related to FAdV-8b FAdV-11 have been increased in chickens China since 2015. Clinical concurrent infections FAdV-4, FAdV-8b, are quite common, yet there no commercially available trivalent vaccines against infection these three serotypes. In our previous study, a bivalent vaccine based on recombinant which fiber-1 was replaced with the fiber has developed. this novel rFAdV-4-fiber/8b + 11 constructed inserting gene into 1966-bp deletion region genome. The vitro replication ability similar parental FAdV-4. One dose immunization inactivated generated robust immune responses FAdV-11, provided efficient clinical protection challenge. This study provides strategy for developing potential prevention control HHS IBH.